Species:

Human

Alternative Name:

IDDM2; ILPR; INS; Insulin; IRDN; MODY10; proinsulin

Uniport ID:

P01308

Assay Type:

Sandwich

unit:

μIU/m

Standard:

100μIU/mL

Sensitivity:

0.95 μIU/mL

Detection Range:

1.56-100μIU/mL

Assay Time:

3.5H

Sample Type:

Serum, Plasma, Tissue homogenate and Other biological samples；Sample Volume=100μL

Research:

Enzyme & Kinase;

Test Principle:

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human INS. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human INS and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human INS, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human INS.You can calculate the concentration of Human INS in the samples by comparing the OD of the samples to the standard curve.